A half-dozen horses entered in this year’s Kentucky Derby Presented by Yum! Brands field made their initial starts without race-day furosemide (also known as Salix), but for Saturday’s 1 1/4-mile classic all six—along with every other horse in the field—will receive the diuretic used to prevent exercise-induced pulmonary hemorrhage (EIPH).

The group of six includes Derby morning-line favorite Orb, who was not given Salix (also referred to as Lasix) in any of his four juvenile starts. The other four U.S.-based horses who started their careers without receiving race-day Salix are Java’s War, Frac Daddy, Will Take Charge, and Oxbow. Salix is allowed to be administered on race days in the United States and all five U.S.-based horses eventually were moved to race-day Salix.

The sixth horse in the group is Lines of Battle, who has made most of his starts in Europe and Dubai, where race-day Salix is prohibited. He will receive Salix for the first time before the Derby after six previous starts without it, including his off-the-board finish in the 2012 Breeders’ Cup Juvenile Turf. For the first time in last year, Breeders’ Cup prohibited race-day Salix in its races for juveniles.

Last year many leading owners pledged to race their juveniles Salix-free, including Orb’s owner-breeders Stuart Janney III and Phipps Stable