Quick Detection of Contagious Equine Metritis
French researchers report they have developed a rapid, effective test for detecting the bacterium that causes the venereal disease contagious equine metritis (CEM). Taylorella equigenitalis is a Gram-negative bacterium that’s responsibl
French researchers report they have developed a rapid, effective test for detecting the bacterium that causes the venereal disease contagious equine metritis (CEM). Taylorella equigenitalis is a Gram-negative bacterium that’s responsible for CEM, which causes infertility and abortions in mares and infertility in stallions. Contagious equine metritis is considered a foreign animal disease in the United States, but it has afflicted horses here via imported stallions and semen.
According to the researchers, who work at the Laboratoire d’Etudes et de Recherches en Pathologie Equine, IPC, in Dozule, France, “The bacteria may be detected in equine genital swabs without need for a preliminary step of DNA extraction or bacterial isolation” in about four hours rather than the one to six days it takes for other types of testing.
According to Dr. Sandrine Petry, lead researcher on the study, bacterial isolation of T. equigenitalis can take four to six days. “In France, we must return a negative result only after six days of culture,” she said. “And it’s forbidden to make the bacterial isolation after 36 hours of swab realization.” This means that the sample cannot be tested 36 hours after the swab was taken, so if it is delayed in reaching the laboratory, the swab might have to be discarded.
She went on to explain that inmmunoflourescence, another diagnostic method, takes one to two days, and in France, diagnosticians “must return a negative result in the three days after the reception of the swab for analysis.” DNA extraction directly on the swab and polymerase chain reaction testing (PCR) takes one day, and PCR directly on the swab without DNA extraction (the new method described in this paper) takes “four hours and half the cost compared with DNA extraction directly on the swab and PCR,” said
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