New Test Could Improve EZL Diagnosis in Working Equids

The new test could lead to faster, low-cost diagnosis and enable better treatment and management.
Share
Favorite
Close

No account yet? Register

ADVERTISEMENT

Endemic in parts of Africa, epizootic lymphangitis (EZL) is a devastating and poorly understood fungal disease with severe health and welfare consequences in working equids. But newly developed tests could lead to faster, low-cost diagnosis and enable better management.

British and Ethiopian researchers teamed up to trial a molecular diagnostic test that would detect the presence of the fungus Histoplasma capsulatum var. farciminosum (HCF) in blood or pus that oozes from EZL wounds. This new DNA-based test provides more accurate results in much less time than do standard laboratory culture methods and could be adapted for surveillance in endemic regions. It should eventually become far less expensive as well, said Claire Scantlebury, BSc, BVSc, PhD, MRCVS, of the University of Liverpool’s School of Veterinary Science, in the U.K.

“At present, the findings presented in this paper show that it is possible to bypass the culture of equine Histoplasma (a notoriously difficult and time-consuming process that can take up to six weeks to gain diagnostic confirmation) and diagnose the infection directly from clinical samples of pus and, for the first time, equine blood, using nested polymerase chain reaction (PCR) methodology,” she said.

Scantlebury and her fellow researchers evaluated the test’s efficacy on 29 horses with clinical signs of EZL and 20 horses without. The blood test confirmed HCF infection in 25 of the sick horses (meaning the other four might not have had HCF circulating in their blood due to the stage of the disease or the presence of another disease with similar signs), and it even detected HCF in two horses that were not (yet) symptomatic, she said

Create a free account with TheHorse.com to view this content.

TheHorse.com is home to thousands of free articles about horse health care. In order to access some of our exclusive free content, you must be signed into TheHorse.com.

Start your free account today!

Already have an account?
and continue reading.

Share

Written by:

Passionate about horses and science from the time she was riding her first Shetland Pony in Texas, Christa Lesté-Lasserre writes about scientific research that contributes to a better understanding of all equids. After undergrad studies in science, journalism, and literature, she received a master’s degree in creative writing. Now based in France, she aims to present the most fascinating aspect of equine science: the story it creates. Follow Lesté-Lasserre on Twitter @christalestelas.

Related Articles

Stay on top of the most recent Horse Health news with

FREE weekly newsletters from TheHorse.com

Sponsored Content

Weekly Poll

sponsored by:

When do you vaccinate your horse?
293 votes · 293 answers

Readers’ Most Popular

Sign In

Don’t have an account? Register for a FREE account here.

Need to update your account?

You need to be logged in to fill out this form

Create a free account with TheHorse.com!